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Hong Kong
Personne àcontacter Mr. Sanjay
Room 704,Bright Way Tower, 33 Mong Kok Road, Kowloon,, Hong Kong
Rotavirus is the primary causative agent of acute gastro-enteritis, especially in children less than 2 years old. Its discovery in ***3 and its association with infantile gastro-enteritis represented a very important advance in the study of gastro-enteritis not caused by acute bacterial infection. Rotavirus is transmitted by oral-faecal contact with an incubation period of **3 daysetc. The Adenovirus is the second most common cause of viral gastro-enteritis in children (****5%). This virus may also cause respiratory diseases and, depending on the serotype, also diarrhoea, conjunctivitis, cystitis,etc. At least *7 serotypes of adenovirus have been described, all sharing a common hexon antigen.Serotypes *0 and *1 are the ones associated with gastro-enteritis, whose main symptom is diarrhoea thatmay last between 9 and *2 days associated with temperature and vomits. SUMMARY AND PRINCIPLE OF THE TEST The test uses new homogenous immunochromatographic system with gold particules. It is a ready to use test which only needs a faecal sample dilution with the supplied ready to use dilution buffer. Specificity is ensured by using a monoclonal antibody conjugated with gold particules and directed against specific human genus-specific Rotavirus antigen or Adenovirus antigens. The immunochromatographic stick is coated with a monoclonal immunoreagent specific for genus-specific Adenovirus hexon antigens or rotaviurs VP6 antigen. Liquid sample and gold conjugate both migrate by capillarity and reach the first specific anti-Adenovirus monoclonal reagent. If Adenovirus is present in the sample, it is blocked and immunoreaction appears as a red-pink line. As sample still migrates, it reaches the second non specific anti-mouse IgG which gives rise to a second red-pink line. This rear line indicates that the chromatography has been developped without hindrance. It appears also with negative samples.
